FMS-Mouse Metabolic Treadmill System

Dedicated system for measuring the metabolic costs of exercise

FMS-Mouse Metabolic Treadmill System

Dedicated system for measuring the metabolic costs of exercise

Features        Description       Specs        Downloads       Contact Us       Sable Enables

Features      Description     Specs      Downloads     Contact Us     Sable Enables

Features

  • Measures O2, CO2 and H2O in treadmill exercise studies, with data acquisition and sub-sample flow control and measurement
  • Industry highest resolution for mouse treadmill metabolic measurements 
  • Automated self-leveling ensures incline accuracy
  • Mouse-activated auditory, blue light and shock stimuli as well as manual brush stimulus
  • Programmable incline, speed, and acceleration
  • Chamber design eliminates user air contamination 
  • Optimized for metabolic measurement with no dead space
  • Includes several high contrast backgrounds to enhance video recording 
  • Expedata graphical analysis software for routine data acquisition and analysis

DESCRIPTION

Dedicated System for Measuring the Metabolic Costs of Exercise

The FMS-Mouse Metabolic Treadmill System is a high-performance, dedicated solution designed specifically for the metabolic measurement of mice. It consists of the Sable Metabolic Treadmill, Field Metabolic System (FMS), and Expedata Software. The system is a stand-alone solution for induced exercise studies such as graded or progressive exercise tests.

Flexible Design

The treadmill provides exceptional flexibility to choose how to design your experiment. You have fine control of speed, acceleration, and incline as well as wide adjustable ranges for each parameter. These parameters are changed via the treadmill controller box, eliminating the need to manually adjust the treadmill. Unique to the Sable Metabolic Treadmill design is automatic chamber leveling to ensure accurate incline and trusted results. Four stimulus choices are available, including electric shock, light, sound, and a manually-operated push brush. Clear chamber windows and insertable backgrounds for contrast enhancement optimize video recordings. 

To clean the treadmill, simply lift the chamber off the treadmill for full access to the running belt. The running belt, chamber, and the two removable waste trays may be cleaned with routine animal husbandry cleaning products. 

Mouse stimuli includes shock grid and LED lights, sound and manual push brush.

Programmable

The user can program and store up to fifteen unique exercise profiles, with each profile containing up to 45 individual changes in speed, inclination and acceleration. The intensity and duration of the voltage, light, and sound stimuli are also programmable and can be set to run in combination or individually. These automated stimuli trigger when the mouse steps on the shock grid. 

Programming is performed with the treadmill controller box through a user-friendly touch screen interface via gloved hand, stylus or by loading preset templates. Animal metadata can be included in the datafiles for traceability. 

Superior Metabolic Measurement

The treadmill uniquely synchronizes changes in a mouse’s metabolism (VO2 – rate of oxygen consumption, RER – respiratory exchange ratio, and EE – energy expenditure) in response to changes in speed and/or inclination. Pull-mode respirometry and optimal air flow design eliminates air-circulation artifacts generated by the moving belt. Care is taken to ensure the incurrent airstream is not altered by the researcher’s respiration. High air flow rate (3.5 liters per minute) and the superior performance of Sable analyzers deliver the industry best metabolic measurement accuracy and resolution, allowing you to see what others miss. 

Applications

The FMS-Mouse Metabolic Treadmill System enables cardiorespiratory fitness tests analogous to human fitness tests. These include sub-maximal and maximal tests to assess VO2max. Tests may be single or multistage. Short test duration enables many tests to be completed per day. The high-performance gas analyzers resolve rapid respirometry changes to quantify the degree of change in response to changing speed or incline (including changes in fuel utilization). Semi-quantitative assessment of fuel utilization is determined by the respiratory exchange ratio.

Real-time energy expenditure (EE), speed and incline results for a single mouse during a maximal exercise capacity test using the FMS-Mouse Metabolic Treadmill System. Data provided by the lab or Dr. Ishan Roy, MD, PhD, at Shirley Ryan Ability Lab and Northwestern University.

Animal Safety

The treadmill is designed with animal safety as a priority. The shock grid is programmable and cannot exceed 5 mA current. Sable test runs used only 1 mA and required only one or two sessions to train naive animals to run with consistent rhythm. The sound and/or light stimulus provide the researcher notification when the mouse steps on the shock grid and the stabilized belt allows for an emergency stop with access to the experimental animal within 2 seconds. 

Treadmill Bridge

Integrated by Sable’s Treadmill Bridge (TMB), the FMS-Mouse Metabolic Treadmill System provides measurement of O2, CO2, and H2,O in treadmill exercise studies, with data acquisition and subsample flow control and measurement.

ExpeData Software

ExpeData allows the user to acquire and explore Treadmill and FMS data in a rich graphic environment where each parameter of the system is merged and displayed in real time. ExpeData’s advanced data analysis tools can be automated for the routine evaluation of multiple data sets.

System Configuration

specs

Treadmill Specifications

Programmable Speed 0 to 100 m/min
Programmable Incline -20 to 40°
Acceleration 0-100 m/min2 in 0.1 m/min2 increments
Chamber Volume 3.75 L
Air Flow Rate for Metabolic Measurement 3.5 L/min
Profiles 15 programmable run profiles, max 45 steps in each
Data Recording

FMS Internal:  SD Card
External: Expedata and PC
Operating Temperature 4° to 45° Celsius
Integration Sable Treadmill Bridge provides integration of Mouse Metabolic Treadmill with FMS and Expedata software
Manual Stimulus Push Brush
Stimulus Settings Shock, Light, Sound
Shock Grid: 0 to 5 mA adjustable in 0.1 mA increments; duration: 5 to 500 milliseconds
Sound range: 70 to 85 dB and a frequency of 5kHz
Light range: 20 lux to 14000 lux at a wavelength of 450-475nm blue light flash
Treadmill Dimensions 12.5” height x 19.1” width x 10.0” depth (31.75cm x 48.5cm x 25.4cm)
Treadmill Weight < 30 pounds (< 13.6kg)

Patent Pending

FMS Specifications

Sensor Type O2 fuel cell; CO2 dual wavelength, non-dispersive infra-red; H2O thin-film capacitive; External Temperature type T thermocouple
Oxygen Sensor Life 2 years in normoxia, typical
Range O2 0-100%; CO2 0-5%; H2O 0-100% RH; Temperature 0-100° C
Accuracy O2 0.1% typical, 2-100%; CO2 1% of reading typical over range of 0-5%; H2O 1% typical 0-95% RH, better than 2% 95-100%; Temperature 0.2˚ C
Resolution O2 0.001%; CO2 0.0001%-0.01% depending on concentration; H2O 0.001% RH, 0.01°C dewpoint
Drift O2 at constant temperature and [O2] <0.02% per hr; CO2 at constant temperature and O% [CO2] <0.001% per hr; H2O at constant temperature with constant N2 flow < 0.01% RH/hr 
Weight FMS 9 lbs (4 kg); Expansion Box 2 lbs (1 kg)
Dimensions FMS L: 14 in (35 cm), W: 12in (30 cm),
H: 6 in (15 cm); Expansion Box L: 7in (17 cm),
W: 7 in (17 cm), H: 3 in (7 cm)
Power Requirements 12-15 VDC nominal, 225-850mA consumption, universal AC adapter supplied
Flow Rate 10-1,500 mL/min
Flow Accuracy 2% of reading, typical
Flow Resolution 0.1 mL/min from 0-99.9 mL/min, 1 mL/min above 100 mL/min
Analog Inputs Eight bipolar analog inputs for voltage standard
Analog Outputs Eight 0-5V outputs, for user-configurable ranges
Digital Data Outputs RS-232 from USB port, SableBus from 5-pin DIN
Digital control outputs 8 TTL logic signals
Internal Data Storage SD Card (up to 32 GB)
Recording Interval User selectable from 0.5 sec to 1 hr
Operating Conditions 3-50° C, non-condensing
Storage conditions 3-60° C

ExpeData Software

ExpeData-P
Computer requirements
  • Computer or processor:
 1 Gigahertz (GHz) or faster x86-bit or x64-bit processor
  • Operating system:
 Windows XP, Windows Vista, Windows 7, Windows 8, Windows 8.1, or Windows 10
  • Storage space:
 Minimum 7.5 Mb for program files (excludes data file storage)
  • Memory:
 2GB or higher
  • Interface:
 Serial port or USB for port emulation required for acquisition (Serial to USB Adapter provided)
Acquisition features (requires additional hardware):
  • Analog interface:
 12 channels
  • Maximum channels:
 96
  • Maximum samples:
 Unrestricted*
  • Event markers:
 Unrestricted*
  • Digital control outputs:
 8, TTL
  • Maximum digital control events:
 Unrestricted*
  • Voltage outputs:
 2, 12-bit (0-5V)
  • Digital data acquisition:
 Yes (requires included plug-in programs)
  • Device regulation utility:
 Yes
  • Serial output for device control:
 Yes
  • Maximum file remarks:
 1,000,000*
*Up to allowable system and virtual memory
Analysis features:
  • Input data formats:
 SCCF (ExpeData), ASCII text, Clipboard
  • Data section selection:
 Manual, automated by marker, automated by criterion
  • Automatable data selection:
 Lowest, highest, and most level sections
  • Data transformations:
 Linear models, non-linear models, general expressions using multiple channels
  • Drift correction:
 Linear and non-linear models, automatable
  • Span correction:
 Yes (to user provided value)
  • Statistics on selected data:
 Mean (with std. dev., SEM, N, etc.), area, linear regression (with slope, intercept, std. errs.)
  • Basic statistical tools:
 Yes (incorporated into spreadsheet)
  • Data export to other formats:
 Comma delimited text, tab delimited text, direct to Excel
  • Macro utility:
 Unrestricted length, up to 20 distinct scripts per macro file

downloads

FMS-Mouse Metabolic Treadmill System Product Note PDF
VO2 max Application Note PDF
FMS Product Note PDF

contact us

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