Industry highest resolution for mouse treadmill metabolic measurements
Automated self-leveling ensures incline accuracy
Mouse-activated auditory, blue light and shock stimuli as well as manual brush stimulus
Programmable incline, speed, and acceleration
Chamber design eliminates user air contamination
Optimized for metabolic measurement with no dead space
Includes several high contrast backgrounds to enhance video recording
Real-time experiment monitoring by Promethion Live software
Measuring the metabolic costs of exercise
The Sable Metabolic Treadmill is an enclosed, single chamber treadmill designed specifically for the metabolic measurement of mice. The treadmill optimizes metabolic measurement with a wide range of settings for inclination, speed, and acceleration—including four options for stimulating the mouse to exercise. Integrated with the Promethion Core metabolic system and Promethion Live software, the treadmill provides real-time system monitoring and graphing of experimental data.
Mouse stimuli includes shock grid and LED lights, sound and manual push brush.
The treadmill provides exceptional flexibility to choose how to design your experiment. You have fine control of speed, acceleration, and incline as well as wide adjustable ranges for each parameter. These parameters are changed via the treadmill controller box, eliminating the need to manually adjust the treadmill. Unique to the Sable Metabolic Treadmill design is automatic chamber leveling to ensure accurate incline and trusted results. Four stimulus choices are available, including electric shock, light, sound, and a manually-operated push brush. Clear chamber windows and insertable backgrounds for contrast enhancement optimize video recordings.
To clean the treadmill, simply lift the chamber off the treadmill for full access to the running belt. The running belt, chamber, and the two removable waste trays may be cleaned with routine animal husbandry cleaning products.
Incline adjustment from -20 to +40 degrees with black high contrast background.
The user can program and store up to fifteen unique exercise profiles, with each profile containing up to 45 individual changes in speed, inclination and acceleration. The intensity and duration of the voltage, light, and sound stimuli are also programmable and can be set to run in combination or individually. These automated stimuli trigger when the mouse steps on the shock grid. Programming is performed with the treadmill controller box through a user-friendly touch screen interface via gloved hand, stylus or by loading preset templates. Animal metadata can be included in the datafiles for traceability.
Superior Metabolic Measurement
The treadmill uniquely synchronizes changes in a mouse’s metabolism (VO2 – rate of oxygen consumption, RER – respiratory exchange ratio, and EE – energy expenditure) in response to changes in speed and/or inclination. Pull-mode respirometry and optimal air flow design eliminates air-circulation artifacts generated by the moving belt. Care is taken to ensure the incurrent airstream is not altered by the researcher’s respiration. High air flow rate (3.5 liters per minute) and the superior performance of Promethion Core analyzers deliver the industry best metabolic measurement accuracy and resolution, allowing you to see what others miss.
The Mouse Respirometry Treadmill enables cardiorespiratory fitness tests analogous to human fitness tests. These include sub-maximal and maximal tests to assess VO2max. Tests may be single or multistage. Short test duration enables many tests to be completed per day. The high-performance gas analyzers resolve rapid respirometry changes to quantify the degree of change in response to changing speed or incline (including changes in fuel utilization). Semi-quantitative assessment of fuel utilization is determined by the respiratory exchange ratio.
Quantitative fuel utilization is possible when the Promethion Core metabolic system is coupled to our Stable Isotope Analyzer. This analyzer enables the quantitation of the catabolism of specific fuels (such as glucose, fructose, and palmitic acid) in addition to the standard respirometry measurements.
Real-time VCO2, VO2, EE, RER, Speed and Incline shown for a single mouse as recorded by a Promethion Core system connected to the Mouse Metabolic Treadmill.
The treadmill is designed with animal safety as a priority. The shock grid is programmable and cannot exceed 5 mA current. Sable test runs used only 1 mA and required only one or two sessions to train naive animals to run with consistent rhythm. The sound and/or light stimulus provide the researcher notification when the mouse steps on the shock grid and the stabilized belt allows for an emergency stop with access to the experimental animal within 2 seconds.
Promethion Core® is the only metabolic and behavioral phenotyping system that enables you to tightly synchronize metabolic data with behavioral events. You will see every aspect of metabolism and behavior more clearly, with finer detail, quality, and repeatability—giving you complete confidence in your data.
For a custom configuration, contact us and our specialists will help you configure the right system for your specific research needs.
15 programmable run profiles, max 45 steps in each
Internal: SD card
External: Promethion Live
4° to 45° Celsius
Full integration with Promethion Core metabolic phenotyping system
Shock, Light, Sound
Shock Grid: 0 to 5 mA adjustable in 0.1 mA increments;
duration: 5 to 500 milliseconds
Sound range: 70 to 85 dBand a frequency of 5kHz
Light range: 20 lux to 14000 lux at a wavelength of 450-475nm
blue light flash
12.5” height x 19.1” width x 10.0” depth (31.75cm x 48.5cm x 25.4cm)