MAVEn™ System for Small Insect Flow-Through Respirometry

Multiplexed metabolism measurements of up to 16 Drosophila or other small animals

MAVEn™ System for Small Insect Flow-Through Respirometry

Multiplexed metabolism measurements of up to 16 Drosophila or other small animals

Features        Description       Specs        Downloads       Contact Us       Sable Enables

Features      Description     Specs      Downloads     Contact Us     Sable Enables

Features

  • Maintains 16 simultaneous individual air flows from 5 mL/min to 200 mL/min
  • Selects the air flow from each chamber in succession and directs that air flow to external gas analyzers
  • Six external voltages can be measured by a high resolution (16 bit) analog-to-digital converter
  • Fully programmable for all aspects of operation
  • Can operate under computer control or as a stand-alone instrument
  • Records data to a computer or to an internal SD card
  • Data files can be loaded directly into Sable Expedata or Microsoft Excel for further analysis
  • Animal activity level can be monitored

DESCRIPTION

Automated metabolic phenotyping of 16 Drosophila in a benchtop system

The Sable MAVEn™ system is an integrated, compact 16-channel flow generator for small insect flow-through respirometry. It provides metabolic measurement of up to 16 animals. 

Compact and cost-effective, the MAVEn packs unprecedented metabolic measurement power and versatility into a slim, easy-to-use instrument.

Metabolic Measurement 

MAVEn™ maintains 16 simultaneous, individual air flows that can be regulated over the range of 5 mL/minute up to 200 mL or more/minute. Each flow is pushed into a sealed container holding an experimental animal. The system automatically selects the air flows from each chamber in succession, with optional baseline interleaving, and directs that air flow to external gas analyzers. Approximately matched flow rates are maintained through all channels simultaneously, minimizing the likelihood of hypoxia or hypercapnia within the chambers. 

The voltage output from each analyzer is measured by a high resolution (16 bit) analog-to-digital converter. Up to 6 external voltages can be monitored and voltages can be transformed to user-defined units. Gas analyzers may include O2, CO2, H2O, or any other detectable gases. Animal activity levels, barometric pressure, ambient temperature and light levels can also be monitored. 

Data Management and Reporting 

MAVEn is fully programmable for all aspects of its operation and can operate under computer control or as a stand-alone instrument. The system can record data to a computer, or to an internal SD card. The data are date and time stamped using an accurate internal real-time clock. MAVEn files can be loaded directly into Sable Systems ExpeData or Microsoft Excel for further analysis.

Second-by-second activity of a single fly over a 10-hour period in a multiplexed arrangement of N=16 flies. Activity monitoring and VCO2 demonstrate the MAVEn’s ability to identify periods of rest and activity and how VCO2 closely tracks activity levels. Further, the data show how activity changes with photoperiod.

specs

Inlet air Supplied by user; pressure (2 kPa up to 200 kPa) depends on maximum flow rate desired; may be tank or pumped air of any composition; optional pump available 
Number of simultaneous push flow streams 16 animal channels plus baseline 
Flow rate range 5 mL/minute to 200 mL/minute, mass flow, precisely controlled via a PID loop 
Flow rate accuracy 2% of reading + 1 mL/minute typical 
Dwell time per animal Programmable from 15 seconds to 3 hours 
Baseline duration Programmable from 15 seconds to 3 hours 
Baseline interleaving Programmable from every chamber switch to every 16 chamber switches 
Animal activity detection Available as optional accessory; recorded with temperature and light level in the data stream 
SD card support SD card (up to 32 GB) data saved as
date- and time-stamped .csv files 
Real time clock Accurate to 1 minute/year
Analog inputs 6 inputs; 16 bit resolution (1 part in 65,536); range -5V to +5V; typically used to capture analog data from user-supplied gas analyzers
Analog input connections BNC female sockets 
Barometric pressure measurement Resolution 1 Pa (0.001 kPa); Accuracy 0.05% 
Light level 0.1 to 5000 Lux 
Ambient temperature measurement Resolution 0.01 deg C, Accuracy ±0.25 deg C
Digital communications (modified RS-232 multidrop) or spooling at
1 sec intervals
Power 10 – 15 VDC at < 1 Amp

downloads

Maven Brochure

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