- Maintains 16 simultaneous individual air flows from 5 mL/min to 200 mL/min
- Selects the air flow from each chamber in succession and directs that air flow to external gas analyzers
- Six external voltages can be measured by a high resolution (16 bit) analog-to-digital converter
- Fully programmable for all aspects of operation
- Can operate under computer control or as a stand-alone instrument
- Records data to a computer or to an internal SD card
- Data files can be loaded directly into Sable Expedata or Microsoft Excel for further analysis
Automated metabolic phenotyping of 16 Drosophila in a benchtop system
The Sable MAVEn™ system is an integrated, compact 16-channel flow generator for small insect flow-through respirometry. It provides metabolic measurement of up to 16 animals.
Compact and cost-effective, the MAVEn packs unprecedented metabolic measurement power and versatility into a slim, easy-to-use instrument.
MAVEn™ maintains 16 simultaneous, individual air flows that can be regulated over the range of 5 mL/minute up to 200 mL or more/minute. Each flow is pushed into a sealed container holding an experimental animal. The system automatically selects the air flows from each chamber in succession, with optional baseline interleaving, and directs that air flow to external gas analyzers. Approximately matched flow rates are maintained through all channels simultaneously, minimizing the likelihood of hypoxia or hypercapnia within the chambers.
The voltage output from each analyzer is measured by a high resolution (16 bit) analog-to-digital converter. Up to 6 external voltages can be monitored and voltages can be transformed to user-defined units. Gas analyzers may include O2, CO2, H2O, or any other detectable gases. Animal activity levels, barometric pressure, ambient temperature and light levels can also be monitored.
Data Management and Reporting
MAVEn is fully programmable for all aspects of its operation and can operate under computer control or as a stand-alone instrument. The system can record data to a computer, or to an internal SD card. The data are date and time stamped using an accurate internal real-time clock. MAVEn files can be loaded directly into Sable Systems ExpeData or Microsoft Excel for further analysis.
Second-by-second activity of a single fly over a 10-hour period in a multiplexed arrangement of N=16 flies. Activity monitoring and VCO2 demonstrate the MAVEn’s ability to identify periods of rest and activity and how VCO2 closely tracks activity levels. Further, the data show how activity changes with photoperiod.
|Inlet air||Supplied by user; pressure (2 kPa up to 200 kPa) depends on maximum flow rate desired; may be tank or pumped air of any composition; optional pump available|
|Number of simultaneous push flow streams||16 animal channels plus baseline|
|Flow rate range||5 mL/minute to 200 mL/minute, mass flow, precisely controlled via a PID loop|
|Flow rate accuracy||2% of reading + 1 mL/minute typical|
|Dwell time per animal||Programmable from 15 seconds to 3 hours|
|Baseline duration||Programmable from 15 seconds to 3 hours|
|Baseline interleaving||Programmable from every chamber switch to every 16 chamber switches|
|Animal activity detection||Available as optional accessory; recorded with temperature and light level in the data stream|
|SD card support||SD card (up to 32 GB) data saved as
date- and time-stamped .csv files
|Real time clock||Accurate to 1 minute/year|
|Analog inputs||6 inputs; 16 bit resolution (1 part in 65,536); range -5V to +5V; typically used to capture analog data from user-supplied gas analyzers|
|Analog input connections||BNC female sockets|
|Barometric pressure measurement||Resolution 1 Pa (0.001 kPa); Accuracy 0.05%|
|Light level||0.1 to 5000 Lux|
|Ambient temperature measurement||Resolution 0.01 deg C, Accuracy ±0.25 deg C|
|Digital communications||(modified RS-232 multidrop) or spooling at
1 sec intervals
|Power||10 – 15 VDC at < 1 Amp|