Stable Isotope Gas Analyzer

Synchronize specific nutrient utilization measurements with total metabolism measurements

Stable Isotope Gas Analyzer

Synchronize specific nutrient utilization measurements with total metabolism measurements

Features        Description       Specs        Downloads       Contact Us       Sable Enables

Features      Description     Specs      Downloads     Contact Us     Sable Enables

Features

  • Simultaneous measurement of 13CO2, C18O2, CO2, and H2O
  • One analyzer can be multiplexed with up to 8 cages
  • Fast response time and low power requirement
  • Wide measurement ranges for δ13C (-100‰ to 4000‰) and CO2 (300 ppm to 25,000 ppm)
  • High precision for both δ13C (0.15‰) and δ18O (1.0‰) as well as CO2 (0.05 ppm) and H2O (50 ppm)
  • Over 10,000 different isotopically-labeled tracers commercially available
  • No consumables or external calibration required

DESCRIPTION

Continuous measurement of 13C and 18O isotopes in exhaled breath

The new Stable Isotope Gas Analyzer is a cutting-edge upgrade to Promethion Core systems, allowing simultaneous measurement of stable isotope tracers synchronously with the Promethion data stream.

Measure the oxidation of exogenous nutrients

Figure A shows the oxidative disposal of a 2.5 mg bolus of 13C-glucose in a control mouse.  Possible experimental manipulations include: age, diet, hormonal treatment, experimental drugs, microbiome manipulations, exercise, thermal exposure, surgical procedures, illness/injury, etc.  Critical metrics include magnitude and duration of response and AUC for calculating % dose recovery.

Measure the oxidation of endogenous nutrients

Figure B shows the oxidation of a fatty acid tracer (13C-Palmitic acid) infused into rodent diet for 10 days, selectively enriching the body lipids with 13C.  The CO2 and δ13C measured during rest, treadmill exercise (15 m/min) and recovery show that total lipid oxidation of a mouse increases during the first minute of exercise, but decreases to resting levels during steady-state exercise.  Researchers could also selectively enrich the proteins in the body by feeding rodent diet infused with 13C-1-L-Leucine, thereby allowing quantitative assessment of endogenous protein oxidation in real-time.

For more information on stable isotope labeling utilization, see:

McCue, M. D. (2011). “Tracking the oxidative and non-oxidative fates of isotopically labeled nutrients in animals.” BioScience 61(3): 217-230.

Welch Jr, K. C., et al. (2016). “Combining respirometry with stable isotopes to investigate fuel use in animals.” Annals of the New York Academy of Sciences 1365(1): 15-32.

McCue, M. D. and K. C. Welch Jr (2016). “13C-Breath testing in animals: Theory, applications, and future directions.” J Comp Physiol 186B(3): 265-285. 

View map of Promethion Core and Promethion Room Calorimetry academic installations

specs

Precision
(1 sec/10 sec/100 sec)		 δ13C: 3.0‰ / 1.0‰ / 0.30‰ 
δ18O: 16‰ / 5‰ / 2‰ 
12CO2: 0.8 ppm / 0.30 ppm / 0.10 ppm 
H2O: 200 ppm (60 sec) / 100 ppm (300 sec) 
Total Uncertainty < 1%
Measurement Range
(meets all specs)		 CO2: 380 – 25000 ppm 
H2O: 4000 – 60000 ppm 
Operational Range H2O: 0 – 70000 ppm (non-condensing) 
CO2: 0 – 50000 ppm
Measurement Rates User-selectable rates up to 1 Hz 
Sampling Conditions Ambient Humidity: non-condensing (0 – 100% RH) 
Sample Temperature: -20 – 50 °C 
Operating Temperature: 0 – 45 °C 
Fittings Outlet (internal pump): 1/4 in.
Inlet: 3/8 in. 
Outputs Digital (RS-232), Ethernet, USB 
Power Requirements 115/230 VAC, 50/60 Hz 66 W 
Dimensions (H x W x D) 18cm (7”) x 47 cm (18.5”) x 36 cm (14”) 
Weight 17 kg (37.5 lbs)

downloads

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