- Simultaneous measurement of 13CO2, C18O2, CO2, and H2O
- One analyzer can be multiplexed with up to 8 cages
- Fast response time and low power requirement
- Wide measurement ranges for δ13C (-100‰ to 4000‰) and CO2 (300 ppm to 25,000 ppm)
- High precision for both δ13C (0.15‰) and δ18O (1.0‰) as well as CO2 (0.05 ppm) and H2O (50 ppm)
- Over 10,000 different isotopically-labeled tracers commercially available
- No consumables or external calibration required
Continuous measurement of 13C and 18O isotopes in exhaled breath
The new Stable Isotope Gas Analyzer is a cutting-edge upgrade to Promethion Core systems, allowing simultaneous measurement of stable isotope tracers synchronously with the Promethion data stream.
Measure the oxidation of exogenous nutrients
Figure A shows the oxidative disposal of a 2.5 mg bolus of 13C-glucose in a control mouse. Possible experimental manipulations include: age, diet, hormonal treatment, experimental drugs, microbiome manipulations, exercise, thermal exposure, surgical procedures, illness/injury, etc. Critical metrics include magnitude and duration of response and AUC for calculating % dose recovery.
Measure the oxidation of endogenous nutrients
Figure B shows the oxidation of a fatty acid tracer (13C-Palmitic acid) infused into rodent diet for 10 days, selectively enriching the body lipids with 13C. The CO2 and δ13C measured during rest, treadmill exercise (15 m/min) and recovery show that total lipid oxidation of a mouse increases during the first minute of exercise, but decreases to resting levels during steady-state exercise. Researchers could also selectively enrich the proteins in the body by feeding rodent diet infused with 13C-1-L-Leucine, thereby allowing quantitative assessment of endogenous protein oxidation in real-time.
For more information on stable isotope labeling utilization, see:
McCue, M. D. (2011). “Tracking the oxidative and non-oxidative fates of isotopically labeled nutrients in animals.” BioScience 61(3): 217-230.
Welch Jr, K. C., et al. (2016). “Combining respirometry with stable isotopes to investigate fuel use in animals.” Annals of the New York Academy of Sciences 1365(1): 15-32.
McCue, M. D. and K. C. Welch Jr (2016). “13C-Breath testing in animals: Theory, applications, and future directions.” J Comp Physiol 186B(3): 265-285.
(1 sec/10 sec/100 sec)
|δ13C: 3.0‰ / 1.0‰ / 0.30‰|
|δ18O: 16‰ / 5‰ / 2‰|
|12CO2: 0.8 ppm / 0.30 ppm / 0.10 ppm|
|H2O: 200 ppm (60 sec) / 100 ppm (300 sec)|
|Total Uncertainty||< 1%|
(meets all specs)
|CO2: 380 – 25000 ppm|
|H2O: 4000 – 60000 ppm|
|Operational Range||H2O: 0 – 70000 ppm (non-condensing)|
|CO2: 0 – 50000 ppm|
|Measurement Rates||User-selectable rates up to 1 Hz|
|Sampling Conditions||Ambient Humidity: non-condensing (0 – 100% RH)|
|Sample Temperature: -20 – 50 °C|
|Operating Temperature: 0 – 45 °C|
|Fittings||Outlet (internal pump): 1/4 in.|
|Inlet: 3/8 in.|
|Outputs||Digital (RS-232), Ethernet, USB|
|Power Requirements||115/230 VAC, 50/60 Hz 66 W|
|Dimensions (H x W x D)||18cm (7”) x 47 cm (18.5”) x 36 cm (14”)|
|Weight||17 kg (37.5 lbs)|