Description
High-Throughput Optogenetics Studies on Flies
Optogenetics is the use of genetic engineering and optics to selectively monitor or control nerve cell activity through light. In Drosophila, transgenic methods are widely used to allow cell-specific gene expression of light-sensitive proteins. These proteins enable scientists to modulate neuronal activity by exposing transgenic flies to specific wavelengths of light. This then helps to assign cause-effect relationships to associated changes in physiology
or behavior.
The FLIC OptoLid is one such peripheral device that provides optical stimulation to individual flies in individual wells in a flexible and programmable fashion, including a variety of open- and closed-looped designs. This lid works with and extends the capability of our FLIC system (version 3) which enables the study of Drosophila feeding behaviors.
When a particular light is coupled with flies expressing certain channelrhodopsins, one can achieve rapid neuronal activation or inhibition. As proof of principle, we created transgenic flies to express channelrhodopsins in neurons that express neuropeptide (npf+ neurons) and tested open loop and closed loop activation of npf+ neurons and monitored feeding interactions.
- Open-loop configuration: Optogenetic activation of npf+ neurons, known to be involved in encoding hunger in insects, independent of feeding behavior increased feeding interactions.
- Closed-loop configuration: Activation of npf+ neurons triggered during or immediately after a fly showing a feeding interaction, increased feeding behaviors by order of two magnitudes, suggesting npf+ neurons’ additional role in reward behavior.
12-well chamber OptoLid
6-well chamber OptoLid
May, Christina E., et al. “High dietary sugar reshapes sweet taste to promote feeding behavior in Drosophila melanogaster.” Cell reports 27.6 (2019): 1675-1685.
Munneke, Allyson S., et al. “The serotonin receptor 5-HT2A modulates lifespan and protein feeding in Drosophila melanogaster.” Frontiers in Aging 3 (2022): 126.
